Natural Product Isolation (1) - Extraction (2024)

Natural Product Extraction: Obtaining Molecules From Organisms

“Let’s plant catnip”, they said.

“It will keep away mosquitoes”, they said.

Natural Product Isolation (1) - Extraction (1)

[from r/pics. h/t @curiouswavefn]

In the previous post on some notable molecules from nature, we saw manyexamples of familiar and interesting compoundsthat can be isolated from various plants, animals, bacteria and even insects. The key pointisthat organisms are veritable chemical factories, and these molecules can have notable uses as medicines, perfumes, flavourants, andmany other commercial applications.

To the uninitiated, however, the process by which we go from a plant or other organism to a sample of a pure molecule mightseem mysterious. For instance, how might we go about:

  • Isolating the active ingredient of catnip (nepetalactone) from leaves? (to make a personal cat army, for instance)
  • Isolating the sex pheromones from a deer tarsal gland? (to bag some trophy bucks?)
  • Obtaining pure caffeine from coffee beans?

You don’t have to be Pablo Escobar to imagine other possibilities, but the above examples are enough toconveythe general idea. In this post, we’ll describea commonfirst step in obtaining natural products from their parent organisms, a process generally calledextraction,as well as a second popular method calledsteam distillation.This is by no means a complete treatment of natural product extraction, by the way – just a sketch of two illustrativemethods. [ For a good time, I highly recommend checking out the Journal of Chemical Ecology, Journal of Natural Products, Natural Product Updates and Natural Product Reports if you want to go down this very fascinating rabbit-hole. ]

Table of Contents

  1. Solvent Extraction
  2. Preliminary Analysis of the Crude Extract
  3. Steam Distillation
  4. Application: Isolation of 99% Nepetalactone from Catnip
  5. Conclusion: Natural Product Extraction

1. Solvent Extraction

The idea behind extraction is pretty simple. We take the organic material in question, pulverize or grind it up (to maximize exposed surface area) and then add an appropriatesolvent. After an appropriate length of time, possibly accompanied by heat and/or agitation, we can then filter off the insoluble material, leaving us with an extract.

If this sounds vaguely familiar, it should! In fact, it’s quite possible you did an extractiontoday.

Natural Product Isolation (1) - Extraction (2)

Obviously, if you’re planning to drink the dilute extract, your choice of solvents is limited to water and perhaps ethanol (useful for extracting absinthe from wormwood, for instance). However, if you’retrying to isolate a organic compound with a fairly low boiling point, using water won’t get you very far (solubility). Instead, organic solvents with low boiling points such as diethyl ether, petroleum ether (short-chain alkanes) and dichloromethane (a.k.a. “methylene chloride”) are commonly used for this purpose, since they are easily removed by a rotary evaporatorwithout significant losses of other volatile components.

Some examples of this process from the primary literaturemake for interesting reading.

From this 1969 Nature paper on the isolation of male black-tailed deer tarsal gland pheromone:

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From this 1971 paper on the isolation of the housefly sex attractant:

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Or the isolation ofthe sex attractant from the pink bollworm moth (a destructive pest of cotton plants)

Natural Product Isolation (1) - Extraction (5)Natural Product Isolation (1) - Extraction (6)

[Killing 850,000 moths might seem like overkill, but it was actually “just right”-kill : this study resulted in the isolation of a mere 1.6 mg (!) of the active pheromone, which was just enough to allowfor determinationof its structure. Two years later, it was then synthesizedon larger scale from commercially available feedstocks and then used in the field for pest control, in lieu of pesticides. That’s the value of total synthesis: it allows us to produce “natural products” without having to harvest them from organisms. ]

The three examples above are illustrative, and have the advantage of simplicity. In cases where extracts are tested for biological activity (e.g. ability to kill various cancer cell lines) a more complex series of extractions are often performed after the initial one, involving adjusting the pH to separate acidic and basic components. One example (often used for bacterial extracts) is a Kupchan scheme.

2. Preliminary Analysis of The Crude Extract

If we’re (extremely) lucky, the concentrated extract will be a reasonably pure sample of one molecule. Much more commonly, however, the crude will be a complex mixture of compounds that requires further separation.

Purification of crude mixtures is a big topic – we’ll leave this for the next post. However, we can briefly mention a pair of related techniques that can quickly reveal the complexity of crude mixtures:liquid chromatography-mass spectrometry (LCMS)Natural Product Isolation (1) - Extraction (7)

and gas chromatography-mass spectrometry (GCMS). Without going into too much detail at the moment, chromatography allows for the separation of acrude mixture into its individual components, and mass spectrometry (MS) determines the mass of each of these individual components.

To use a rough analogy, it’s a bit like afootrace where the runners begin as a cluster at the starting line and are separatedby the end of the race according to how fast they have run down the track. Once separated, they can be characterized by mass spectrometry: enough studies have been done of enough naturally occurring compounds that the MS data can be checked against commerciallyavailable databases to give a preliminary ID of various components of the crude.

As an example, on the left is an example of the GCMS from the deer tarsal gland (from the paper cited above). Every peak represents a discrete compound [this is late 1960’s technology, by the way – current techniques are significantly more refined]. Inspection reveals the presence of at least 15 compounds in the crude.

3. Steam Distillation

A second popular method for separating natural products from their parent organisms is a technique called steam distillation. It has the advantage of being cheap, does not require organic solvents, and is even practicable in thekitchen. Here, the organic material (e.g. ground up leaves, skin, seeds) is boiled vigorously in water and the steam is collected and condensed into a separate flask. The organic-soluble material (an oil) can then be collected as a thin layer floating on top of the condensed distillate.It’s also a common technique in undergraduate teaching labs – for example, in this preparation of limonene from orange peel:

One of the additional advantages of this technique is that oils with fairlyhigh boiling points can be obtained under relatively mild conditions. For instance, notethat limonene has a boiling point of 176°C, but steam (100°C) is being used to distill it off. [If the two liquids were miscible, we’d distill off the water fraction first, leaving the oil behind: but since the two liquids are immiscible, the composition of the vapour phase ispurelya function of temperature].

4. An Application of Steam Distillation: 99% Pure Nepetalactone from Catnip

This extremely well-doneInstructable shows how to prepare pure nepetalactone from Nepataleaves via steam distillation, using materials one would find in your kitchen (you can see the whole thing after you close the annoying popup window).

Natural Product Isolation (1) - Extraction (8)

The nepetalactoneobtained from this method turned out to be extremelypure: about 99% by GCMS, even more pure than some commercially available catnip oil extracts.

Natural Product Isolation (1) - Extraction (9)

[Just in case you’re wondering, the GCMS was not a kitchen instrument, but the procedure itself is very DIY-friendly].

Steam distillation can also be used to prepare, for example, oil of lavender, rose oil, clove oil, caraway, and many others. It’s one of the most rewarding applications of kitchen chemistry.

5. Conclusion: Natural Product Extraction

The point of this article was merely to illustrate a few examples of isolating natural products, or natural product extracts from various organisms in order to render the process more concrete. In the next post, we’ll discuss some options forpurifying those extracts so that we can actually start to talk about the analysis of individual compounds.

Notes

Related Articles

  • A Gallery of Some Interesting Molecules From Nature
  • Structure Determination Case Study: Deer Tarsal Gland Pheromone
  • Natural Product Isolation (2) – Purification Techniques, An Overview
  • Now Available – The Spectroscopy Pack (PDF)
  • Infrared Spectroscopy: A Quick Primer On Interpreting Spectra
  • IR Spectroscopy: 4 Practice Problems
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